Rabbit prothrombin fragment 1+2 (F1+2) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔凝血酶原片段F1+2(F1+2)酶联免疫试剂盒（CSB-EQ027672RB）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F1+2含量。兔凝血酶原片段F1+2（F1+2）是凝血酶原分子的氨基末端片段，当凝血酶被激活时释放，具有多肽特性，半衰期约90分钟。其在凝血过程中通过检测F1+2水平，可反映凝血酶的活性，是评估凝血功能的重要指标。试剂盒检测范围为0.312 pmol/mL-20 pmol/mL，常用于血栓形成机制、抗凝药物作用评估等研究；支持科研人员探究心血管疾病模型、凝血功能障碍动物实验中的F1+2动态变化；为凝血与纤溶系统调控、药物干预效果分析等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

F1+2
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 pmol/mL-20 pmol/mL
灵敏度：

0.078 pmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	98
	Range %	92-104
1:4	Average %	97
	Range %	93-101
1:8	Average %	87
	Range %	82-95

回收率：

The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-92
EDTA plasma (n=4)	92	85-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.