Human prothrombin fragment 1+2,F1+2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

95

Range %

89-99

1:2

Average %

98

Range %

93-102

1:4

Average %

86

Range %

81-93

1:8

Average %

92

Range %

88-97

The recovery of human F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

93

90-96

EDTA plasma (n=4)

87

83-90

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/L

OD1

OD2

Average

Corrected

2

2.296

2.274

2.285

2.137

1

1.445

1.498

1.472

1.324

0.5

0.916

0.936

0.926

0.778

0.25

0.538

0.568

0.553

0.405

0.125

0.376

0.398

0.387

0.239

0.06

0.270

0.291

0.281

0.133

0.03

0.218

0.216

0.217

0.069

0

0.146

0.149

0.148