Rat procalcitonin (PCT) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠降钙素原（PCT）酶联免疫检测试剂盒（货号：CSB-E13419r），采用双抗体夹心法原理，可定量分析大鼠血清、血浆及组织匀浆样本中的PCT浓度。PCT是一种与细菌感染及系统性炎症反应密切相关的蛋白质，在科研中常用于评估感染性疾病模型的炎症程度及病理机制。该试剂盒检测范围覆盖31.25 pg/mL至2000 pg/mL，最低检测限为31.25 pg/mL，操作流程简便，通过标准曲线与样本吸光度值的比对实现精准定量，适用于体外实验中对不同来源样本的PCT水平检测。研究者可借助该试剂盒探索炎症相关疾病的分子机制、细菌感染模型的动态变化，或评估抗炎药物、免疫调节剂的干预效果。本产品仅限科学研究使用，实验全程无需复杂前处理，配套试剂及操作说明完备，可为感染免疫学、药理学等领域的实验提供可靠数据支持。
缩写：

PCT
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	96
	Range %	91-103
1:4	Average %	97
	Range %	91-105
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of rat PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-107
EDTA plasma (n=4)	97	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.