Pig Procalcitonin,PCT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪降钙素原(PCT)酶联免疫试剂盒（CSB-E15004p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCT含量。PCT（降钙素原）是一种蛋白质。它健康状态下在体内水平极低，感染时会显著升高。其研究机制主要围绕作为感染诊断和病情监测的生物标志物，在细菌感染时，机体产生炎症反应促使PCT合成增加，可辅助判断感染严重程度与预后。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于科研场景中猪类疾病模型的炎症进程监测、疫苗或药物干预效果评估、免疫调控机制研究等方向，为兽医科学、动物医学研究及生物标志物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PCT
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

39 pg/mL-2500 pg/mL
灵敏度：

9.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	95
	Range %	88-98
1:4	Average %	92
	Range %	89-96
1:8	Average %	93
	Range %	88-98

回收率：

The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	96	92-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.