Canine Procalcitonin(PCT)ELISA Kit

产品详情

产品描述：

犬降钙素原（PCT）是一种与细菌感染密切相关的炎症标志物前体蛋白，在犬类免疫应答研究中具有重要价值。本试剂盒基于夹心法酶联免疫原理，可定量检测血清、血浆及细胞培养上清液中的犬PCT蛋白，检测灵敏度范围为46.88-3000 pg/mL，适用于微量样本的高精度分析。试剂盒采用高特异性抗体对目标蛋白进行双位点识别，通过优化的反应体系确保数据的稳定性和可重复性。其标准化操作流程兼容常规实验室设备，96孔板设计支持批量样本的高效检测。在科研领域，该产品可用于探索犬类感染性疾病模型的病理机制、评估抗生素治疗过程中炎症反应动态变化，或作为免疫调节药物开发的生物标志物检测工具，为兽医基础研究及实验动物科学提供可靠的定量分析解决方案。
缩写：

PCT
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates
检测范围：

46.88 pg/mL-3000 pg/mL
灵敏度：

11.72 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	95
	Range %	91-99
1:4	Average %	91
	Range %	85-97
1:8	Average %	83
	Range %	80-86

回收率：

The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-93
EDTA plasma (n=4)	104	101-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.