Mouse Procalcitonin,PCT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠降钙素原（PCT）酶联免疫试剂盒（货号：CSB-E10371m），采用双抗体夹心法定量检测血清、血浆、细胞培养上清液及组织匀浆样本中的PCT含量，检测灵敏度达1.56 pg/mL，线性范围为1.56-100 pg/mL，适用于科研场景中PCT相关机制研究。PCT是机体响应细菌感染或全身性炎症时由甲状腺外组织释放的重要生物标志物，其水平变化可反映炎症反应程度及病理进程。该试剂盒基于高特异性配对抗体构建，能够精准识别小鼠PCT分子，操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应，结果可通过酶标仪在450 nm波长下读取吸光值进行定量分析。科研人员可将其应用于实验动物模型中脓毒症、全身性感染及炎症性疾病的机制探索，或用于评估药物干预对炎症通路的影响。本试剂盒支持多种生物样本类型检测，为实验室开展PCT动态监测、病原体-宿主互作研究及免疫调节实验提供可靠工具，所有操作需遵循体外研究规范。
别名：

N/A
缩写：

PCT
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	88-93
1:40	Average %	102
	Range %	99-104
1:80	Average %	94
	Range %	89-96
1:160	Average %	97
	Range %	94-101

回收率：

The recovery of mouse PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.