Rat Substance P,SP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠P物质（SP）酶联免疫试剂盒（货号：CSB-E08358r），能够定量检测血清、血浆、细胞培养上清液及组织匀浆中的SP含量。P物质是一种广泛分布于神经系统和胃肠道的神经肽，参与疼痛信号传递、炎症反应调控及肠道运动调节等生理病理过程。试剂盒基于双抗体夹心法原理设计，检测灵敏度为6.25 pg/mL，线性范围覆盖6.25 pg/mL至400 pg/mL，适用于体外样本中SP蛋白的精准定量分析。其高特异性与稳定性可满足科研领域对神经生物学机制、炎症相关疾病模型或胃肠道功能研究的检测需求，例如通过分析不同实验条件下动物模型血清或组织中的SP表达水平，探索其在神经信号传导或免疫调节中的作用。本产品操作便捷，配套试剂齐全，实验流程标准化，可为科研人员提供可靠的实验数据支持，仅限科学研究使用。
别名：

N/A
缩写：

SP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	98-106
1:4	Average %	92
	Range %	87-95
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.