Pig Substance P(SP)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪P物质（SP）酶联免疫检测试剂盒（货号CSB-E16385p），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的SP浓度。P物质作为神经递质和神经调节肽，广泛分布于中枢及外周神经系统，参与痛觉传递、炎症反应及组织修复调控等生理病理过程，是神经免疫交叉研究的重要靶点。试剂盒检测灵敏度达0.25 ng/mL，线性范围覆盖0.25-16 ng/mL，通过预包被高特异性抗体实现精准捕获，配套优化缓冲体系确保实验重复性，适用于探究猪模型中SP在神经退行性疾病、肠道功能紊乱或炎症相关机制中的动态变化。实验流程包含标准品梯度稀释、样本预处理及显色反应，96孔板设计支持批量检测，操作便捷且兼容常规酶标仪分析。本产品专为科研场景开发，可为动物模型构建、药效评估或分子通路研究提供可靠数据支持。
别名：

N/A
缩写：

SP
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.25 ng/mL-16 ng/mL
灵敏度：

0.06 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	93
	Range %	90-96
1:4	Average %	95
	Range %	91-99
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	89	86-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.