Mouse Substance P,SP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠P物质（Substance P，SP）酶联免疫检测试剂盒（货号CSB-E08359m），采用双抗体夹心法定量检测样本中SP蛋白浓度。SP是一种广泛分布于中枢和外周神经系统的神经肽，参与痛觉传导、炎症调节及神经免疫应答等生理过程，是研究神经源性炎症、疼痛机制及神经退行性疾病的重要生物标志物。试剂盒适用于血清、血浆、细胞培养上清及组织匀浆等样本类型，检测灵敏度高，线性范围为3.12-200 pg/mL，可精准分析低浓度样本。实验操作便捷，通过预包被特异性捕获抗体与标记检测抗体的协同作用，结合显色反应实现目标蛋白定量。该试剂盒广泛用于基础科研领域，如探究神经系统疾病模型中SP的分泌动态、评估药物对神经肽表达的调控作用，或解析炎症反应与神经信号通路的关联机制，为神经生物学、免疫学及药理学研究提供可靠工具。
别名：

N/A
缩写：

SP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-104
1:200	Average %	94
	Range %	89-98
1:400	Average %	89
	Range %	86-96
1:800	Average %	94
	Range %	89-97

回收率：

The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	86	82-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1:200

产品评价：我用CSB-E08359m检测正常血清，其od值为0.7/0.604/0.559/0.639/0.577/0.671

By 张老师