Human Substance P,SP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人p物质（Substance P，SP）酶联免疫检测试剂盒（货号：CSB-E08357h），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本分析。p物质作为速激肽家族的重要神经递质，广泛分布于中枢及外周神经系统，参与疼痛信号传递、炎症调控及胃肠功能调节等生理病理过程。本试剂盒灵敏度高，检测线性范围为6.25-400 pg/mL，可稳定识别样本中低浓度SP分子。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，配合标准曲线实现精准定量。该试剂盒适用于神经生物学、免疫调节机制等基础研究，特别在疼痛模型评估、神经炎症研究或胃肠道功能实验中，可为探索SP在病理生理过程中的动态变化提供可靠工具。试剂盒含标准品、检测抗体、显色液等全套组分，操作流程优化至4小时内完成，兼容常规酶标仪检测。
别名：

N/A
缩写：

SP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	102
	Range %	98-105
1:4	Average %	89
	Range %	85-96
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of human SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-92
EDTA plasma (n=4)	85	81-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.