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中文名称：大鼠前列腺素E2(PGE2)酶联免疫试剂盒
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货号：CSB-E07967r
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：CUSABIO定量检测大鼠前列腺素E2（PGE2）的酶联免疫试剂盒（货号：CSB-E07967r），适用于血清、血浆、组织匀浆及细胞裂解液等多种样本类型。PGE2作为花生四烯酸代谢通路的重要产物，广泛参与炎症反应调控、疼痛信号传导及组织修复等生理病理过程，其水平检测对炎症相关疾病机制研究具有重要意义。试剂盒采用竞争法检测原理，检测灵敏度达0.5 pg/mL，线性范围为0.5-100 pg/mL，可精准量化样本中低浓度PGE2含量。该试剂盒适用于体外实验研究，例如探究炎症模型中PGE2的动态变化、评估药物或化合物对前列腺素代谢通路的影响，以及组织微环境中脂质介质的定量分析等科研场景，为炎症机制研究、药效学评价及生物标志物探索提供高效检测工具。
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缩写：PG-E2
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates, cell lysates
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检测范围：0.5 pg/mL-100 pg/mL
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灵敏度：0.25 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 91-99 1:2 Average % 92 Range % 87-96 1:4 Average % 93 Range % 89-98 1:8 Average % 105 Range % 99-109 -
回收率：
The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-99 EDTA plasma (n=4) 101 96-104 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 100 0.228 0.224 0.226 25 0.365 0.348 0.357 8 0.560 0.539 0.550 2 0.813 0.787 0.800 0.5 1.258 1.234 1.246 0 2.031 2.176 2.104 -
数据处理：
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货期：3-5 working days
引用文献
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- Pinus densiflora Root Extract Attenuates Osteoarthritis Progression by Inhibiting Inflammation and Cartilage Degradation in Interleukin-1β and Monosodium Iodoacetate-Induced Osteoarthritis Models YM Park, DY Shin, HY Lee, HM Hwang, JG Kim,Nutrients,2024
- Cannabigerol as an anti-inflammatory agent altering the level of arachidonic acid derivatives in the colon tissue of rats subjected to a high-fat high-sucrose diet K Sztolsztener, E Harasim-Symbor,Biomedicine & Pharmacotherapy,2024
- N-acetylcysteine acts as a potent anti-inflammatory agent altering the eicosanoid profile in the development of simple steatosis and its progression to hepatitis K Sztolsztener,/,2023
- Arbutin attenuates CFA-induced Arthritis by modulating expression levels of 5-LOX, NF-κB, IL-17, PGE-2 and TNF-α A Malik,researchsquare,2023
- α-Lipoic acid–A promising agent for attenuating inflammation and preventing steatohepatitis in rats fed a high-fat diet M Zwierz,Archives of biochemistry and biophysics,2023
- Thymol ameliorates 5‐fluorouracil‐induced intestinal mucositis: Evidence of down‐regulatory effect on TGF‐β/MAPK pathways through NF‐κB LA Al‐Khrashi,Journal of biochemical and molecular toxicology,2023
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- The Effect of the Mixed Extract of Kalopanax pictus Nakai and Achyranthes japonica Nakai on the Improvement of Degenerative Osteoarthritis through Inflammation Inhibition in the Monosodium Iodoacetate-Induced Mouse Model HY Lee,Current Issues in Molecular Biology,2023
- Therapeutic effect of cannabidiol on myocardial arachidonic acid content in various lipid fractions in a rat model of obesity K Sztolsztener,Prostaglandins & other lipid mediators,2023
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- Anti-inflammatory Action of YHQ by Regulating 5-LOX/COX-2/NF-κB/MAPKs/Akt Signaling Pathways in RAW 264.7 Macrophage cells Hu TY, et al,Journal of Herbal Medicine,2019
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- Ginsenoside metabolite compound K exerts anti-inflammatory and analgesic effects via downregulating COX2 Jingyu Chen.et al,Inflammopharmacology,2018
- Gastro protective Effect of Pentagalloyl Glucose on Aspirin Induced Gastric Mucosal Ulcer in Comparison with Omeprazole, Famotidine and Melatonin Moharram.et al,Journal of Pharmaceutical Sciences and Research,2017
- Enhanced lipoxygenase/LTD4 signaling accounts for the exaggerated hypertensive and nephrotoxic effects of cyclosporine plus indomethacin in rats Helmy MW.et al,Biomedicine & Pharmacotherapy,2018
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- Nebivolol prevents indomethacin-induced gastric ulcer in rats. El-Ashmawy NE.et al,J Immunotoxicol.,2016
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- Geraniol ameliorates TNBS-induced colitis: Involvement of Wnt/ß-catenin, p38MAPK, NFkB, and PPARr signaling pathways Soubh AA. et al,Life Sci,2015
- Rhizoma Paridis Saponins Suppresses Tumor Growth in a Rat Model of N-Nitrosomethylbenzylamine-Induced Esophageal Cancer by Inhibiting Cyclooxygenases-2 Pathway. Yan S. et al,PLoS One,2015
- Reduction of spinal PGE 2 concentrations prevents swim stress-induced thermal hyperalgesia Guevara C et al,Neurosci Lett,2015
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- Mechanisms underlining gender differences in Phenylephrine contraction of normoglycaemic and short-term Streptozotocin-induced diabetic WKY rat aorta U.I. Aloysius et al,Brain Research,2012
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最新研究进展：PGE2是一种前列腺素，与炎症和癌症等多种疾病有关。近期的研究表明，PGE2可以在体内诱导肝癌干细胞分化为肝癌细胞，从而促进肝癌的发生和发展。此外，研究人员还发现，通过靶向PGE2的合成酶和受体可以减少肿瘤的生长和转移，从而为肿瘤治疗提供新的策略。
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