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中文名称：人前列腺素E2(PGE2)酶联免疫试剂盒
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货号：CSB-E07965h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO人前列腺素E2（PGE2）酶联免疫检测试剂盒（货号：CSB-E07965h），基于竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本的科研检测。PGE2作为花生四烯酸代谢通路的重要活性物质，在炎症反应、疼痛调节及细胞增殖分化中发挥关键作用，其水平变化常与心血管疾病、肿瘤微环境等研究领域密切相关。本试剂盒检测灵敏度覆盖1.5 pg/mL至300 pg/mL的线性范围，采用预包被特异性抗体的微孔板设计，通过样本中游离PGE2与酶标抗原竞争结合抗体位点，结合显色反应实现目标物浓度测定，操作流程包含样本预处理、孵育、洗涤及比色读数等标准化步骤。该试剂盒可为体外研究炎症介导机制、免疫调节通路、药物干预效果评估等提供可靠数据支持，特别适用于实验室条件下探索生物样本中PGE2的动态变化规律，满足细胞实验、动物模型或离体组织分析等基础科研需求，实验全程需配套标准品及酶标仪进行定量分析。
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缩写：PG-E2
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：1.5 pg/mL-300 pg/mL
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灵敏度：0.75 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 91-99 1:2 Average % 92 Range % 86-96 1:4 Average % 97 Range % 92-103 1:8 Average % 84 Range % 80-88 -
回收率：
The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-98 EDTA plasma (n=4) 90 83-94 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 300 0.193 0.201 0.197 75 0.349 0.327 0.338 24 0.523 0.531 0.527 6 0.903 0.962 0.933 1.5 1.316 1.393 1.355 0 2.271 2.212 2.242 -
数据处理：
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货期：3-5 working days
引用文献
- Therapeutic Framework Nucleic Acid Complexes Targeting Oxidative Stress and Pyroptosis for the Treatment of Osteoarthritis J Li, Y Li, X Shang, S Xu, Z Zhang, S Xu, X Wang,Materials Today Bio,2024
- Expression analysis of genes and MicroRNAs involved in recurrent implantation failure: New noninvasive biomarkers of implantation B Habibi,Biomedical and Biotechnology Research Journal (BBRJ),2023
- Prodigiosin/celecoxib-loaded into zein/sodium caseinate nanoparticles as a potential therapy for triple negative breast cancer TU Kwon,Scientific reports,2024
- EPC-exosomal miR-26a-5p improves airway remodeling in COPD by inhibiting ferroptosis of bronchial epithelial cells via PTGS2/PGE2 signaling pathway C Liu,Scientific reports,2023
- Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression Z Liu,Experimental and Therapeutic Medicine,2021
- Anti‑inflammatory and anti‑rheumatic activities in vitro of alkaloids separated from Aconitum soongoricum Stapf Feicui Zhao,Experimental and Therapeutic Medicine,2021
- Analyses of Clinical Features and Investigations on Potential Mechanisms in Patients with Alzheimer’s Disease and Olfactory Dysfunction Zuo, Li-jun.et al,Journal of Alzheimer\'s Disease,2018
- Increase of Prostaglandin E2 in the Reversal of Ductal Constriction After Polyphenol Restriction Vian I.et al,Ultrasound Obstet Gynecol,2017
- Excessive Iron and α-Synuclein Oligomer in Brain are Relevant to Pure Apathy in Parkinson Disease. Wang F.et al,J Geriatr Psychiatry Neurol.,2016
- Effect and Potential Mechanism of Electroacupuncture Add-On Treatment in Patients with Parkinson?? Disease W Fang. et al,Evidence-Based Complementary and Alternative Medicine,2015
- Effect and Potential Mechanism of Electroacupuncture Add-On Treatment in Patients with Parkinson's Disease Fang Wang. et al,Evidence-Based Complementary and Alternative Medicine,2015
- A procedure for efficient non-viral siRNA transfection of primary human monocytes using nucleofection Scherer O et al,J Immunol Methods,2015
- Expression and possible role of non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) in the human endometrium and endometriosis Seok Kyo Seo et al,Hum. Reprod,2010
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