Rabbit Prostaglandin E2,PG-E2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对兔源前列腺素E2（PGE2）的定量检测试剂盒，采用双抗体夹心法原理设计，适用于科研领域分析血清、血浆、组织匀浆及细胞裂解液等多种生物样本。PGE2作为重要的脂类信号分子，广泛参与炎症反应、疼痛感知及免疫调节等生理病理过程，是研究炎症相关疾病机制、药物作用效果及细胞信号转导的关键靶点。试剂盒检测灵敏度达31.25 pg/mL，线性范围为31.25-2000 pg/mL，通过预包被特异性捕获抗体和酶标检测抗体的协同作用，实现对目标分子的高特异性识别与定量分析。该试剂盒可广泛应用于实验动物模型中炎症动态监测、药理学研究中的化合物效能评估，以及体外细胞实验中炎症因子调控机制的探索，为科研人员提供稳定可靠的检测工具。试剂盒操作流程标准化，兼容常规酶标仪进行OD值测定，实验数据可通过标准曲线实现精准定量。
缩写：

PG-E2
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-98
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	90-105
1:8	Average %	97
	Range %	86-98

回收率：

The recovery of rabbit PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-110
EDTA plasma (n=4)	90	80-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.