Canine Prostaglandin E2,PG-E2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬前列腺素E2（PGE2）酶联免疫试剂盒（货号：CSB-E13493c）采用竞争法原理实现定量检测，适用于犬血清、血浆及组织匀浆样本中PGE2的科研分析。PGE2作为前列腺素家族的重要成员，广泛参与炎症反应、疼痛信号传导及组织修复等生理病理过程，在犬类免疫调节、生殖生理及疾病模型研究中具有重要价值。本试剂盒检测范围为10-2000 pg/mL，可精准覆盖生物样本中PGE2的典型浓度水平。实验采用预包被板结构，配套提供标准品、检测抗体、显色底物及终止液，操作流程优化后可在4小时内完成检测。该产品特别适用于研究犬类炎症性疾病（如关节炎、胃肠炎）、免疫应答机制、药物抗炎效果评估，以及生殖系统或肿瘤相关研究中的PGE2动态监测，为科研人员提供稳定可靠的实验工具。检测结果需通过酶标仪在450nm波长下读取，建议采用双波长校正以提高数据准确性。
缩写：

PG-E2
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	89-105
1:4	Average %	93
	Range %	87-100
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-96
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.