Rat Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

产品详情

产品描述：

大鼠血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒（CSB-E04703r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF - AB是一种重要的靶点。它属于血小板衍生生长因子家族，在细胞生长、增殖、迁移和血管生成等过程中有关键作用。目前研究聚焦于其信号通路机制，探索通过阻断该靶点来抑制肿瘤生长、纤维化等疾病发展，为相关疾病治疗提供新思路。试剂盒检测范围为7.8 pg/mL-500 pg/mL，特别适用于大鼠模型研究中PDGF - AB的动态监测，例如创伤修复实验、炎症反应评估或血管生成相关机制研究，为探索PDGF - AB在组织再生、病理进程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-AB
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	88
	Range %	84-93
1:4	Average %	105
	Range %	101-109
1:8	Average %	100
	Range %	95-106

回收率：

The recovery of rat PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	102	97-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.