Rabbit Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒（CSB-E15910Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF-AB是一种血小板衍生生长因子二聚体。其在细胞增殖、分化等生理过程及肿瘤、纤维化等疾病发生发展中起作用。研究机制上，主要通过与受体结合激活下游信号通路，影响细胞行为，目前是疾病治疗靶点研究热点之一。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外实验环境下对生物样本的批量检测，支持细胞生物学、基础医学研究中涉及组织再生、血管病变或肿瘤微环境等领域的 PDGF - AB 动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-AB
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	87
	Range %	83-95
1:4	Average %	94
	Range %	90-98
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of rabbit PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-93
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.