Pig Platelet-Derived Growth Factor AB (PDGF-AB) ELISA kit

产品详情

产品描述：

猪血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒（CSB-EQ027978PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF - AB是一种血小板衍生生长因子，在细胞生长、增殖和分化等过程中起重要作用。其异常表达与肿瘤、纤维化等疾病的发生发展相关。研究机制聚焦于其信号通路，通过干预该信号通路有望为相关疾病的治疗提供新策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于猪模型研究中PDGF - AB的动态监测，例如在创伤修复、心血管疾病机制或干细胞分化等基础科研领域，为探索生长因子网络调控提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-AB
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	90
	Range %	85-99
1:4	Average %	93
	Range %	84-97
1:8	Average %	95
	Range %	83-100

回收率：

The recovery of pig PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-101
EDTA plasma (n=4)	93	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.