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中文名称：大鼠N端前脑钠素(NT-proBNP)酶联免疫试剂盒
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货号：CSB-E08752r
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠N端前脑钠素（NT-proBNP）酶联免疫检测试剂盒（货号：CSB-E08752r），基于双抗体夹心法原理实现定量分析，适用于血清、血浆及组织匀浆样本中NT-proBNP的精准检测。NT-proBNP是由心室肌细胞分泌的脑钠肽前体裂解产物，其水平可敏感反映心脏压力负荷与心室功能异常，是心血管疾病研究中的重要生物标志物。本试剂盒检测范围为7.8-500 pg/mL，可满足低浓度样本的检测需求，适用于心血管疾病机制研究、心肌损伤动物模型评估及药物干预效果分析等科研领域。试剂盒操作流程标准化，支持对大鼠心脏组织或循环系统中的NT-proBNP水平进行动态监测，为心肌肥厚、心力衰竭等模型的病理生理研究提供可靠工具，亦可用于探索心血管相关药物对心室功能的调节作用。
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缩写：NT-proBNP
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：7.8 pg/mL-500 pg/mL
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灵敏度：1.95 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cardiovascular
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 84-95 1:2 Average % 96 Range % 90-101 1:4 Average % 99 Range % 94-105 1:8 Average % 84 Range % 80-88 -
回收率：
The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-94 EDTA plasma (n=4) 94 89-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 500 2.769 2.651 2.710 2.548 250 1.973 1.901 1.937 1.775 125 1.183 1.217 1.200 1.038 62.5 0.793 0.841 0.817 0.655 31.2 0.506 0.550 0.528 0.366 15.6 0.404 0.379 0.392 0.230 7.8 0.261 0.245 0.253 0.091 0 0.165 0.159 0.162 -
数据处理：
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货期：3-5 working days
引用文献
- Shenmai injection revives cardiac function in rats with hypertensive heart failure: involvement of microbial-host co-metabolism L Li, S Zhong, J Ye, S Hu, X Xiong,BMC Complementary Medicine and Therapies,2025
- Clinicopathological Studies on the Impact of Grape Seed Extract and L-Carnitine as Cardioprotective Agents Against Doxorubicin-Induced Toxicity in Rats TS Aldayel, OE Kilany, HNG El-Hak, HMA Abdelrazek,Life,2024
- VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD Y Zhang, Z Shen, Z Mao, D Huang, C Lou,Frontiers in Bioscience-Landmark,2024
- Uncovering the Therapeutic Potential of Lithium Chloride in Type 2 Diabetic Cardiomyopathy: Targeting Tau Hyperphosphorylation and TGF-β Signaling via GSK-3β Inhibition L Abou Assi,Pharmaceutics,2024
- DL0805-1, a novel Rho-kinase inhibitor, attenuates lung injury and vasculopathy in a rat model of monocrotaline-induced pulmonary hypertension D Chen,European journal of pharmacology,2023
- Role of Klotho and AGE/RAGE-Wnt/β-Catenin Signalling Pathway on the Development of Cardiac and Renal Fibrosis in Diabetes B Martín-Carro,International Journal of Molecular Sciences,2023
- Puerarin-V Improve Mitochondrial Respiration and Cardiac Function in a Rat Model of Diabetic Cardiomyopathy via Inhibiting Pyroptosis Pathway through P2X7 Receptors S Sun,International journal of molecular sciences,2022
- Early cardiac-chamber-specific fingerprints in heart failure with preserved ejection fraction detected by FTIR and Raman spectroscopic techniques N Tombolesi,Scientific Reports,2022
- Renal Denervation Mitigated Fecal Microbiota Aberrations in Rats with Chronic Heart Failure Z Guo,Evidence-Based Complementary and Alternative Medicine,2021
- Cardiac-chamber-specific Fingerprints in Heart Failure with Preserved Ejection Fraction. Early Detection of Pathological Conditions by FTIR and Raman Spectroscopic Techniques N Tombolesi,Research Square,2021
- Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation Zhaoyu Li,Evidence-Based Complementary and Alternative Medicine,2020
- The cardiac protection of Baoyuan decoction via gut-heart axis metabolic pathway Z Du,Phytomedicine,2020
- Chronic high-fat diet consumption induces an alteration in plasma/brain neurotensin signaling, metabolic disturbance, systemic inflammation/oxidative stress, brain … N Saiyasit,Neuropeptides,2020
- Rho-Kinase inhibitors ameliorated diclofenac-induced cardiotoxicity in chloroquine-treated adjuvant arthritic rats MH Arafa,Life Sciences,2020
- YQWY decoction reverses cardiac hypertrophy induced by TAC through inhibiting GATA4 phosphorylation and MAPKs Jing-JingHUANG, et al,Chinese Journal of Natural Medicines,2019
- Assessment of Pregabalin-Induced Cardiotoxicity in Rats: Mechanistic Role of Angiotensin 1-7 Awwad Z M, et al,Cardiovascular Toxicology,2019
- Telmisartan and Captopril Ameliorate Pregabalin-Induced Heart Failure in Rats Awwad Z M, et al,Toxicology,2019
- The mechanism of RNA oxidation involved in the development of heart failure Liu T, et al,Free Radical Research,2019
- Blood pressure-targeted stepwise resuscitation for hemorrhagic shock in rats. Lee JH.et al,J Trauma Acute Care Surg.,2014
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