Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

中文名称：

小鼠N端前脑钠素(NT-proBNP)酶联免疫试剂盒
货号：

CSB-E05153m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO定量检测小鼠血清或血浆中N端前脑钠素（NT-proBNP）的酶联免疫试剂盒，采用双抗体夹心法原理，通过高特异性抗体实现精准定量分析，适用于心脏功能相关研究。NT-proBNP是由心肌细胞分泌的神经激素前体裂解产物，其浓度变化可反映心肌张力负荷和损伤程度，在心血管疾病模型构建、药物干预效果评估等科研领域具有重要应用价值。该试剂盒检测灵敏度达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，配套提供预包被96孔板、标准品、检测抗体及显色系统，支持4小时内完成样本检测。实验流程包含标准品梯度稀释、样本孵育（37℃ 1小时）、洗板、抗体结合（37℃ 1小时）、显色反应（37℃ 15分钟）等标准化步骤，适用于心血管基础研究、转基因动物模型评估、心肌损伤机制探索等科研场景。产品经严格验证，批间批内重复性良好，与同类生物样本无交叉反应，可为心衰模型、心肌肥厚等实验研究提供可靠数据支持。
缩写：

NT-proBNP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	96
	Range %	91-105
1:4	Average %	89
	Range %	85-99
1:8	Average %	93
	Range %	83-98

回收率：

The recovery of mouse NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-105
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.235	2.199	2.217	2.090
5	1.884	1.749	1.817	1.690
2.5	1.462	1.375	1.419	1.292
1.25	0.978	1.035	1.007	0.880
0.625	0.552	0.549	0.551	0.424
0.312	0.289	0.284	0.287	0.160
0.156	0.203	0.211	0.207	0.080
0	0.128	0.125	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Does Telotristat Have a Role in Preventing Carcinoid Heart Disease? R Geng,International Journal of Molecular Sciences,2024
Inhibition of miR-128-3p Attenuated Doxorubicin-Triggered Acute Cardiac Injury in Mice by the Regulation of PPAR-γ WB Zhang,PPAR Research,2021
Follistatin-Like 1 Protects against Doxorubicin-Induced Cardiomyopathy through Upregulation of Nrf2 Y Zhao,Oxidative Medicine and Cellular Longevity,2020

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■ 常见问题解答

1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, what is the host.
7. Please let us know whether the antibody and the standard include any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

Thanks for your inquiry.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody, affinity chromatography.
Stop solution is 4N sulfuric acid.
Standard is 293 cell-derived recombinant protein）.
The antibody and the standard contain Preservative：proclin 300(1:3000).
The kit doesn't contain thimerosal or sodium azide or 2-mercaptoethanol etc.
Pls let me know if you have any further questions. Thank you.

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Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

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