Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

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中文名称：

犬N端前脑钠素(NT-proBNP)酶联免疫试剂盒
货号：

CSB-E17577c
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO犬N端前脑钠素（NT-proBNP）酶联免疫检测试剂盒（货号CSB-E17577c），基于夹心法原理实现定量检测，适用于犬血清或血浆样本中心脏功能相关生物标志物的科研分析。NT-proBNP是心肌细胞在心室壁张力升高或心脏负荷过载时分泌的激素前体裂解产物，其浓度变化与心脏功能状态密切相关，常用于评估犬类心肌肥厚、充血性心力衰竭等疾病的病理机制研究。该试剂盒采用预包被特异性抗体的96孔板，通过双抗体夹心法捕获目标蛋白，检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，可精准量化低丰度样本。实验体系包含标准品、检测抗体、HRP标记链霉亲和素及显色底物等核心组分，支持科研人员开展心血管疾病模型构建、药物干预效果评估或病理生理机制研究，为犬类心脏疾病的实验动物模型提供可靠的分子检测工具。试剂盒操作流程标准化，适用于实验室常规检测需求，实验全程无需特殊设备，3.5小时内即可完成检测。
缩写：

NT-proBNP
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	97
	Range %	90-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-100

回收率：

The recovery of canine NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-108
EDTA plasma (n=4)	97	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.675	2.655	2.665	2.516
10	2.152	2.144	2.148	1.999
5	1.311	1.302	1.307	1.158
2.5	0.781	0.799	0.790	0.641
1.25	0.377	0.363	0.370	0.221
0.625	0.264	0.254	0.259	0.110
0.312	0.186	0.190	0.188	0.039
0	0.146	0.152	0.149

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Serum Levels of Cardiac Markers NT-proANP and NT-proBNP in Brachycephalic bitches at Different Gestational Stages Feliciano M.et al,Reprod Domest Anim.,2016

■ 常见问题解答

Per customers request, please provide the following information regarding Canine N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit (CSB-E17577c)
Can this kit be used with cat samples too? Or is this kit only designed to be used for dogs?
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. What is the immunogen of the capture antibody?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen of the detection antibody?

Thanks for your inquiry!
CSB-E17577c
This kit is specific to dog samples. It is not suggested to be used to test other species.
Capture antibody is mouse monoclonal antibody and immunogen is 293 cell-derived recombinant protein(full length).
Detection antibody is goat polyclonal antibody and immunogen is 293 cell-derived recombinant protein(fragment).
Standard is 293 cell-derived recombinant protein. Sorry we can not disclose the sequence.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.