Pig N-terminal pro-brain natriuretic peptide (NT-proBNP) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪N端前脑钠素(NT-proBNP)酶联免疫试剂盒（CSB-EQ027465PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NT-proBNP含量。NT - proBNP即N末端B型利钠肽原，是心室肌细胞分泌的BNP前体裂解产物，主要用于诊断心力衰竭和判断预后。，心衰时心室壁张力增加，刺激NT - proBNP释放，通过检测其血浆水平变化为病情评估提供依据。试剂盒检测范围为7.8 pg/mL-500 pg/mL，猪心血管疾病模型研究、心脏病理机制探索、药物干预效果评估、实验室开展猪心脏疾病模型构建、心肌病机制研究、心血管药物筛选、深入解析心脏疾病的分子调控机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

NT-proBNP
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-108
1:2	Average %	99
	Range %	91-102
1:4	Average %	94
	Range %	86-98
1:8	Average %	98
	Range %	90-102

回收率：

The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-109
EDTA plasma (n=4)	97	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.