Rat Immunoglobulin A,IgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠免疫球蛋白A（IgA）定量检测试剂盒（货号：CSB-E07987r），基于竞争性酶联免疫原理开发，适用于血清和血浆样本的科研分析。IgA是黏膜免疫系统中的关键抗体，广泛参与呼吸道、消化道等部位的病原体防御机制，其水平变化可反映机体免疫状态及炎症反应进程。本试剂盒检测范围覆盖7.81-2000 ng/mL的宽泛浓度梯度，最低检测限达3.9 ng/mL，可精确测定不同生理或实验条件下样本中的IgA含量。试剂盒采用预包被抗体与样本中IgA竞争结合生物素标记抗原的检测模式，通过标准曲线实现定量分析，操作流程包含样本预处理、孵育、洗涤和显色等标准化步骤。该产品适用于免疫学研究、感染性疾病模型建立、疫苗开发评估等科研领域，特别适用于长期监测实验动物体液免疫应答动态，或探究免疫调节剂、营养干预等外界因素对大鼠IgA分泌的影响，为免疫相关机制研究提供可靠的数据支持。
缩写：

IgA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

7.81 ng/mL-2000 ng/mL
灵敏度：

7.81 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	97-104
1:2000	Average %	92
	Range %	88-96
1:4000	Average %	90
	Range %	86-94
1:8000	Average %	103
	Range %	99-107

回收率：

The recovery of rat IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.