Human Immunoglobulin A,IgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人免疫球蛋白A（IgA）酶联免疫试剂盒（货号CSB-E07985h），基于双抗体夹心法原理开发，可定量检测血清或血浆样本中IgA的浓度，检测范围为0.24-1000 ng/mL，具备高灵敏度和宽泛的线性范围。IgA是人体黏膜免疫的关键成分，广泛分布于呼吸道、消化道等黏膜表面及体液中，其浓度变化与免疫调节、感染防御及炎症反应等过程密切相关。CUSABIO试剂盒采用特异性抗体对，通过优化反应体系确保检测准确性，适用于科研场景中免疫机制研究、疾病模型分析或生物样本中IgA水平的动态监测，例如肠道菌群与黏膜免疫关联性研究、慢性炎症性疾病模型的生物标志物检测等。实验操作兼容常规酶标仪检测，支持科研人员高效获取血清或血浆样本中IgA的定量数据，为免疫相关基础研究提供可靠工具。
别名：

N/A
缩写：

IgA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.24 ng/mL-1000 ng/mL
灵敏度：

0.24 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10000	Average %	89
	Range %	85-94
1:20000	Average %	90
	Range %	86-97
1:40000	Average %	96
	Range %	90-103
1:80000	Average %	89
	Range %	86-93

回收率：

The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-94
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

IgA（免疫球蛋白A）是一种免疫球蛋白，分布于人体内许多黏膜表面和体液中。IgA是黏膜免疫的主要保护因子，主要通过抗体中和病原体和调节肠道微生物群维持肠道健康。此外，IgA还在妊娠期间保护胎儿免受感染，参与自身免疫调节，发挥重要的生物学作用。