Bovine Immunoglobulin A,IgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛免疫球蛋白A(IgA)酶联免疫试剂盒（货号：CSB-E12018B），采用竞争法原理实现定量检测，适用于分析牛血清、血浆、细胞培养上清液及组织匀浆样本中IgA的含量，检测灵敏度覆盖0.39 μg/mL至25 μg/mL的宽范围。牛IgA作为黏膜免疫系统的重要成分，在呼吸道、消化道等黏膜屏障的免疫防御中起关键作用，其水平监测对研究牛群免疫状态、疫苗效力评估及肠道健康机制等科研领域具有重要价值。该试剂盒采用标准化的预包被板与优化反应体系，支持科研人员高效完成样本检测，适用于畜牧免疫学研究、疫苗开发验证、病原感染模型建立等场景。试剂盒提供完整的标准品与操作试剂，具备高特异性与稳定性，可在4-6小时内完成检测流程，重复性好且操作便捷，为牛免疫相关基础研究提供可靠工具。本产品严格限定于科学研究用途，不涉及任何临床或诊断应用。
缩写：

IgA
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.39 μg/mL-25 μg/mL
灵敏度：

0.39 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	96
	Range %	92-101
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-94

回收率：

The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	97	92-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.