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  4. Pig Immunoglobulin A,IgA ELISA Kit

Pig Immunoglobulin A,IgA ELISA Kit

文献引用 (25) 评价 (1) 说明书下载
  • 中文名称：
    猪免疫球蛋白A(IgA)酶联免疫试剂盒
  • 货号：
    CSB-E13234p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO试剂盒采用竞争法酶联免疫吸附原理，专为定量检测猪血清或血浆样本中免疫球蛋白A（IgA）设计，适用于科研领域对猪黏膜免疫功能的评估研究。猪IgA作为黏膜免疫系统的重要效应分子，广泛参与呼吸道、消化道等黏膜表面的病原体防御，其浓度变化可反映机体局部免疫状态。试剂盒预包被特异性抗体，实验过程中样本内IgA与标记物竞争结合抗体位点，通过显色反应强度反比计算目标物浓度，检测灵敏度达0.146 μg/mL，线性范围覆盖0.146-37.5 μg/mL，可精准检测微量样本中的IgA含量。试剂盒包含预包被96孔板、系列浓度标准品、生物素标记检测抗体、HRP标记链霉亲和素及显色底物等核心组分，标准品与抗体需-20°C保存，其他组分4°C储存，有效期6个月。该产品适用于畜牧科研中疫苗效力评估、疾病模型建立时的免疫应答监测，或规模化养殖中通过定期检测IgA水平分析猪群健康状态，实验操作需注意避免样本反复冻融或溶血干扰，建议使用新鲜分离的血清/血浆进行检测以保证数据准确性。
  • 缩写：
    IgA
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.146 μg/mL-37.5 μg/mL
  • 灵敏度：
    0.146 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 92  
    Range % 88-94  
    1:2000 Average % 93  
    Range % 90-98  
    1:4000 Average % 88  
    Range % 85-94  
    1:8000 Average % 95  
    Range % 91-100  
  • 回收率：
    The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 103 98-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    37.5 0.199 0.209 0.204    
    9.375 0.445 0.420 0.433    
    2.345 0.924 0.955 0.940    
    0.586 1.413 1.447 1.430    
    0.146 2.101 2.114 2.108    
    0 2.547 2.503 2.525    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：其他 猪

稀释比：1:100

产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。

By 谢老师

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相关问答

■ 常见问题解答
Q:

Hi. I would like to know if this kit is used feces samples from pig. If so, I would like knowing the cost. Also I would like to know if you have avilable the kit with reagents for plate preparation and its respective cost, as well. Thanks much.

A:
Thanks for your inquiry.
For IgA, there are serotype and secretory type. If you want to test feces samples, we recommend you choose Pig secretory immunoglobulin A,SIgA ELISA KitCSB-E12063p. No matter you use CSB-E12063p or CSB-E13234p, we suggest you do a pretest first.
All the components in the kit are well prepared, so you don't need to prepare the reagents by yourself. Sorry we can not provide reagents for plate preparation.Pls let me know if you have any further questions. Thank you.
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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |