Rat Homocysteine(Hcy)ELISA Kit

Rat Homocysteine(Hcy)ELISA Kit

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中文名称：

大鼠高半胱氨酸(Hcy)酶联免疫试剂盒
货号：

CSB-E13376r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒专用于定量检测大鼠血清、血浆及组织匀浆中的高半胱氨酸（Hcy）水平。高半胱氨酸是蛋氨酸代谢过程中的关键中间产物，其异常积累与氧化应激、心血管疾病及神经系统疾病的发生密切相关，是科研领域研究代谢调控、疾病机制的重要生物标志物。试剂盒基于双抗体夹心法原理设计，可精准检测0.78-50 nmol/mL浓度范围内的Hcy含量，灵敏度达0.28 nmol/mL，适用于微量样本分析。产品提供预包被96孔板及标准品、检测抗体等全套组分，操作流程优化，可在3.5小时内完成检测。该试剂盒广泛应用于大鼠模型的基础研究，例如在高血压、动脉粥样硬化或阿尔茨海默病等动物模型中评估Hcy水平变化，或用于探究营养干预、药物代谢对Hcy调控的影响，为疾病机制解析及治疗策略开发提供可靠数据支持。
别名：

N/A
缩写：

Hcy
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 nmol/mL-50 nmol/mL
灵敏度：

0.195 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	94
	Range %	89-97
1:8	Average %	100
	Range %	93-103

回收率：

The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	95	92-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
50	2.874	2.771	2.823	2.670
25	2.223	2.245	2.234	2.081
12.5	1.418	1.389	1.404	1.251
6.25	0.756	0.775	0.766	0.613
3.12	0.462	0.448	0.455	0.302
1.56	0.321	0.313	0.317	0.164
0.78	0.232	0.225	0.229	0.076
0	0.155	0.151	0.153

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.