Mouse Homocysteine(HCY)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠高半胱氨酸（Hcy）酶联免疫检测试剂盒（货号：CSB-E16551m），基于双抗体夹心法原理开发，可对血清、血浆及组织匀浆样本中的Hcy进行定量分析。高半胱氨酸是甲硫氨酸代谢的关键中间产物，其异常积累与氧化应激、心血管疾病及神经退行性疾病的发生密切相关，在基础代谢研究及疾病机制探索中具有重要价值。该试剂盒检测灵敏度高，线性范围为4.7-300 nmol/mL，可精准检测微量样本中的Hcy浓度变化。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立定量曲线，结合显色反应实现目标物浓度测定，适用于小鼠模型中的代谢功能评估、疾病进程监测或药物干预效果分析，例如在动脉粥样硬化、阿尔茨海默病等研究中动态观察Hcy水平变化，或在营养干预实验中探究维生素B族对Hcy代谢的调控作用。本产品提供完整的配套试剂及操作指南，实验全程无需特殊设备，3小时内即可完成检测，为科研人员提供高效可靠的Hcy定量解决方案。
别名：

N/A
缩写：

Hcy
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.7 nmol/mL-300 nmol/mL
灵敏度：

1.1 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	90
	Range %	86-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	99
	Range %	95-107

回收率：

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.