Human Homocysteic acid,Hcy ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人同型半胱氨酸（Hcy）酶联免疫检测试剂盒（货号CSB-E08895h），基于双抗体夹心法定量原理开发，可精确检测血清、血浆、组织匀浆及尿液样本中的Hcy含量。Hcy是甲硫氨酸代谢的重要中间产物，其异常水平与心血管疾病、神经系统退行性病变及代谢功能障碍等病理过程密切相关，已成为生命科学领域广泛关注的研究靶点。该试剂盒检测范围覆盖0.78-50 nmol/mL，最低检测限达0.195 nmol/mL，可灵敏捕捉样本中Hcy的细微浓度变化。实验体系包含预包被可拆卸96孔板、系列标准品、特异性检测抗体、酶结合物及显色底物等完整组分，操作流程优化后可在4.5小时内完成检测。所有组分需-30℃避光保存，有效期6个月。本产品适用于疾病分子机制研究、营养代谢分析、药物干预效果评估及生物标志物筛查等科研场景，为实验室提供标准化、高重复性的Hcy检测方案，仅限科学研究使用。
别名：

N/A
缩写：

Hcy
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, urine
检测范围：

0.78 nmol/mL-50 nmol/mL
灵敏度：

0.195 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-96
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	92	89-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.