Rat Homocysteic acid,Hcy ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒专用于定量检测大鼠血清、血浆及组织匀浆样本中的同型半胱氨酸（Hcy），采用双抗体夹心法原理，通过特异性抗体对靶标分子进行高灵敏度捕获与检测。同型半胱氨酸作为蛋氨酸代谢的关键中间产物，其浓度异常与氧化应激、心血管功能及神经退行性病变等研究领域密切相关。该试剂盒检测范围为0.78-50 nmol/mL，最低可精确检测至0.78 nmol/mL，适用于科研场景下对大鼠模型体内Hcy代谢水平的动态监测，例如营养代谢研究、药物毒性评价或疾病机制探索。试剂盒包含预包被抗体、标准品、检测抗体及显色系统等核心组分，操作流程标准化，兼容常规酶标仪在450 nm波长下读取吸光度值。样本处理建议遵循离心去杂质、分装冻存等规范，组织样本需预先制备为匀浆液。CUSABIO研究者提供稳定可靠的数据支持，可广泛应用于基础医学、药理学及动物模型构建等领域，助力Hcy相关分子机制的深入解析。
别名：

N/A
缩写：

Hcy
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 nmol/mL-50 nmol/mL
灵敏度：

0.195 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-108
1:2	Average %	96
	Range %	92-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	97	94-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.