Rat Complement fragment 5a,C5a ELISA Kit

Rat Complement fragment 5a,C5a ELISA Kit

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中文名称：

大鼠补体片断5a(C5a)酶联免疫试剂盒
货号：

CSB-E08513r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO针对大鼠补体片段5a（C5a）的酶联免疫检测试剂盒（货号：CSB-E08513r），采用双抗体夹心法定量检测血清、血浆、细胞培养上清及组织匀浆中的C5a蛋白水平。C5a是补体活化过程中产生的关键炎症介质，参与趋化免疫细胞、促进炎性因子释放等病理生理过程，其动态变化与感染、自身免疫性疾病及炎症反应调控机制的研究密切相关。试剂盒通过预包被特异性抗体实现目标蛋白捕获，检测灵敏度达1.56 ng/mL，线性范围涵盖1.56至100 ng/mL，可精准量化不同样本中C5a的表达差异。该产品适用于体外研究炎症信号通路激活状态、评估药物对补体系统的干预效果，或分析疾病模型中免疫应答动态变化，例如通过检测实验动物血清中C5a浓度变化揭示炎症反应程度，或利用细胞培养上清数据探究特定刺激对补体活化途径的影响。试剂盒提供标准化操作流程，配套完整组分，支持科研人员在心血管疾病、神经炎症、免疫调节等基础研究领域开展精准的蛋白定量分析。
别名：

N/A
缩写：

C5a
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	102
	Range %	96-108
1:2000	Average %	94
	Range %	91-98
1:4000	Average %	96
	Range %	88-99
1:8000	Average %	92
	Range %	86-98

回收率：

The recovery of rat C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.241	2.140	2.191	2.053
50	1.804	1.703	1.754	1.616
25	1.215	1.204	1.210	1.072
12.5	0.713	0.702	0.708	0.570
6.25	0.464	0.475	0.470	0.332
3.12	0.328	0.339	0.334	0.196
1.56	0.245	0.251	0.248	0.110
0	0.137	0.138	0.138

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.