Rabbit complement fragment 5a,C5a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为科研设计的兔补体片段5a（C5a）酶联免疫检测试剂盒（货号：CSB-E13247Rb），采用双抗体夹心法原理实现定量分析，适用于血清、血浆及组织匀浆样本中C5a蛋白水平的精准检测。C5a是补体系统活化后产生的关键过敏毒素，在炎症反应中发挥趋化免疫细胞、促进血管通透性等重要作用，其异常表达与脓毒症、类风湿性关节炎等疾病机制密切相关。本试剂盒检测灵敏度高，线性范围覆盖0.625 ng/mL至40 ng/mL，可满足不同浓度样本的检测需求，实验操作流程标准化，兼容常规酶标仪进行读数分析。科研人员可将其应用于炎症信号通路研究、免疫调控机制探索、疾病动物模型评估（如感染性疾病或自身免疫模型），以及抗炎药物研发过程中生物标志物的动态监测。试剂盒提供完整的预包被板、标准品及检测试剂，确保实验稳定性和重复性，为体外实验中C5a蛋白水平的定量检测提供可靠工具。
缩写：

C5a
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	92
	Range %	88-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rabbit C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-93
EDTA plasma (n=4)	101	97-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.