Pig complement fragment 5a (C5a) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪补体片段5a（C5a）酶联免疫检测试剂盒（货号：CSB-EQ027637PI），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的C5a蛋白浓度。C5a作为补体系统裂解C5产生的活性片段，在炎症反应、趋化免疫细胞及病原体清除中发挥重要作用。试剂盒检测灵敏度为9.375 ng/mL，线性检测范围覆盖9.375-600 ng/mL，实验全程约需4.5小时，包含预包被特异性抗体的96孔板、标准品、检测抗体及显色底物等核心组分，可通过酶标仪在450 nm波长下读取吸光度值进行定量分析。该试剂盒适用于科研领域对猪源样本中C5a水平的动态监测，例如免疫相关疾病模型研究、感染或炎症状态下补体系统激活程度的评估，以及药物或疫苗开发中免疫调节效应的体外检测，为探索补体介导的病理生理机制提供可靠工具。
缩写：

C5a
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.375 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	91-109
1:2	Average %	87
	Range %	82-93
1:4	Average %	102
	Range %	98-106
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of pig C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	92-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.