Bovine complement fragment 5a (C5a) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专用于定量检测牛血清、血浆或组织匀浆中的补体片段5a（C5a）浓度。C5a是补体系统裂解C5产生的强效趋化因子，在先天免疫应答中通过招募免疫细胞和介导炎症反应发挥关键作用，其水平变化与感染性疾病、过敏反应等病理过程密切相关。试剂盒检测范围覆盖4.69-300 ng/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用实现高精度定量，实验全程仅需3.5小时，样本处理时需注意血浆需EDTA抗凝、组织匀浆需离心取上清液。该产品适用于动物免疫机制研究、炎症性疾病模型构建、疫苗效力评估等科研领域，尤其为反刍动物感染性疾病的免疫病理分析提供可靠工具，实验结果需结合标准曲线通过450nm波长判读。
缩写：

C5a
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.69 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.