Rat C-Peptide ELISA Kit

Rat C-Peptide ELISA Kit

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中文名称：

大鼠C肽(C-Peptide)酶联免疫试剂盒
货号：

CSB-E05067r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中C肽（C-Peptide）的竞争法ELISA试剂盒，检测范围覆盖1.25-20 ng/mL，可灵敏反映样本中C肽水平。C肽是胰岛素原裂解产生的副产物，与胰岛素等摩尔分泌，作为评估胰岛β细胞功能的重要生物标志物，广泛应用于糖尿病发病机制、胰岛功能调控及相关药物干预研究领域。试剂盒采用预包被特异性抗体的微孔板，通过样本中C肽与标记物竞争结合抗体的原理实现定量检测，适用于评估实验动物模型中的胰岛素分泌动态、胰腺组织功能状态及代谢相关研究。样本兼容性覆盖血清、血浆（推荐EDTA抗凝血）及经离心处理的组织匀浆液，操作流程包含标准品梯度稀释、样本孵育、显色反应及酶标仪检测（推荐450nm主波长/630nm参比波长双波长读数）。本试剂盒提供全套标准品、检测缓冲液及终止液，建议实验前通过预实验优化样本稀释比例以确保检测值位于标准曲线线性范围内，为糖尿病研究、药物作用评价及代谢调控机制探索提供可靠的科研工具。
缩写：

C-Peptide
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-20 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C-peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-103
1:2	Average %	90
	Range %	85-94
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat C-peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	96	91-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.155	0.151	0.153
10	0.289	0.304	0.297
5	0.501	0.491	0.496
2.5	0.847	0.912	0.880
1.25	1.954	1.826	1.890

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.