Mouse C-Peptide ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠C肽（C-Peptide）定量检测试剂盒，采用夹心法酶联免疫吸附技术（ELISA），适用于血清和血浆样本的精准检测。C肽是胰岛素原裂解产生的生物标志物，其与胰岛素等摩尔释放入血，可作为评估胰岛β细胞功能的关键指标，在糖尿病模型研究、胰岛素分泌机制探索及代谢调控实验中具有重要价值。本试剂盒检测范围为2.86-50 ng/mL，通过预包被特异性抗体实现高灵敏度检测，操作流程标准化，可在实验室内快速完成样本分析。该产品严格验证了批次间稳定性与特异性，适用于小鼠血清或血浆样本中C肽含量的定量测定，能够为胰岛素相关代谢研究、糖尿病动物模型评估以及药物干预效果分析提供可靠数据支持，满足科研领域对β细胞功能动态监测的需求。
缩写：

C-Peptide
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

3.125 ng/ml-50 ng/ml
灵敏度：

0.625 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	90
	Range %	85-96
1:4	Average %	95
	Range %	87-106
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	91-103