Pig C-Peptide ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪C肽（C-Peptide）酶联免疫检测试剂盒（货号：CSB-E15977p），采用双抗体夹心法定量检测猪血清、血浆或组织匀浆中的C肽水平，检测范围为1.25-80 ng/mL。C肽是胰岛素原裂解为胰岛素时产生的生物标志物，其浓度可间接反映胰岛β细胞功能活性，在糖尿病机制研究、代谢调控模型构建及胰岛功能相关药物开发中具有重要科研价值。试剂盒包含预包被抗体的96孔板、系列浓度标准品、生物素标记检测抗体、链霉亲和素-HRP及显色底物等核心组分，操作流程包括样本孵育、洗涤、显色及终止反应，3-4小时内即可通过酶标仪读取OD值并计算浓度。该试剂盒适用于实验室条件下动态监测猪胰岛功能状态、评估糖尿病动物模型干预效果或探究代谢性疾病相关分子机制，建议样本在-20℃分装保存并避免反复冻融以确保检测稳定性。
缩写：

C-Peptide
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

0.312 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	87
	Range %	81-93

回收率：

The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.