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中文名称:人C肽(C-Peptide)酶联免疫试剂盒
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货号:CSB-E05066h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人C肽(C-Peptide)酶联免疫试剂盒(货号:CSB-E05066h),采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的C肽水平,其线性检测范围覆盖0.5至8 ng/mL。C肽是由胰岛β细胞在胰岛素原分解过程中产生的生物活性片段,其释放量与胰岛素等摩尔,可作为评估胰岛β细胞功能及胰岛素分泌能力的重要生物标志物,在糖尿病发病机制研究、胰岛功能评估及代谢性疾病相关药物开发等领域具有科研价值。本试剂盒针对人源样本设计,具备高特异性和灵敏度,通过预包被特异性抗体实现目标蛋白的精准捕获与检测,实验操作标准化且重复性良好。适用于基础医学研究中动态监测糖尿病模型动物的胰岛功能变化,或体外实验中对细胞分泌C肽水平的定量分析,为探索代谢调控机制、筛选潜在治疗靶点提供可靠数据支持。实验需自备标准品稀释液及常规酶标仪,建议严格按照说明书操作流程执行。
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缩写:C-Peptide
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.5 ng/mL-8 ng/mL
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灵敏度:0.25 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
8
2.306
2.434
2.370
2.187
4
1.495
1.598
1.547
1.364
2
0.788
0.808
0.798
0.615
1
0.341
0.329
0.335
0.152
0.5
0.241
0.228
0.234
0.051
0
0.112
0.120
0.116
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数据处理:
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货期:3-5 working days
引用文献
- The Association of Fried Meat Consumption With the Gut Microbiota and Fecal Metabolites and Its Impact on Glucose Homoeostasis, Intestinal Endotoxin Levels, and Systemic Inflammation: A Randomized Controlled-Feeding Trial Jian Gao,Diabetes Care,2021
- NR4A1 Promotes Diabetic Nephropathy by Activating Mff-Mediated Mitochondrial Fission and Suppressing Parkin-Mediated Mitophagy Sheng J.et al,Cell Physiol Biochem,2018
相关产品
靶点详情
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最新研究进展:C-肽是由胰岛素的前体胰岛素分子在转化成胰岛素过程中被剪切而产生的。虽然C-肽没有胰岛素的生物学活性,但它可以用来反映胰岛素的分泌。近年来,有研究表明C-肽除了反映胰岛素分泌外,还具有自身的生物学作用,如可以促进β细胞增殖和生存、促进脂肪细胞分化、减轻糖尿病肾病等作用。此外,C-肽还被用作糖尿病和胰岛素抵抗的治疗。例如,最近的一项研究表明,在2型糖尿病患者中,静脉注射C-肽可以显著降低血糖、胰岛素剂量和甘油三酯水平,同时增加葡萄糖利用率。
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