Rat Beta-Endorphin,β-EP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清、血浆或组织匀浆中β-内啡肽（β-EP）含量的酶联免疫试剂盒，采用竞争法检测原理。β-内啡肽作为内源性阿片肽家族的重要成员，广泛分布于中枢神经系统与内分泌组织，参与疼痛调节、应激反应及情绪调控等生理过程，是神经内分泌研究的重要靶点。试剂盒检测灵敏度达10 pg/mL，线性范围覆盖10-2000 pg/mL，标准曲线包含7个浓度梯度，适用于检测低浓度样本。实验操作需配备酶标仪（450 nm波长）及37℃恒温孵育设备，试剂盒包含预包被板、检测抗体、标准品及显色底物等核心组分，保存于2-8℃条件下有效期达12个月。该产品可应用于神经生物学研究、疼痛机制探索、应激反应模型评估等科研领域，尤其适合分析不同实验条件下β-EP在体液或组织中的动态变化，为动物模型中的神经递质调控研究提供可靠检测工具。
别名：

beta-Endorphin, beta-EP
缩写：

β-EP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-90
1:2	Average %	97
	Range %	91-105
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	90	84-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.