Human Beta-Endorphin,β-EP ELISA Kit

Human Beta-Endorphin,β-EP ELISA Kit

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中文名称：

人β内啡肽(β-EP)酶联免疫试剂盒
货号：

CSB-E06821h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人β-内啡肽（β-EP）酶联免疫检测试剂盒（货号：CSB-E06821h），采用双抗体夹心法定量检测血清、尿液、脑脊液、唾液及组织匀浆等多种生物样本中的β-EP含量，其检测范围覆盖62.5至4000 pg/mL的线性区间，适用于神经生物学、疼痛机制及应激反应等基础研究领域。β-内啡肽作为内源性阿片肽家族成员，广泛参与痛觉调节、情绪调控和免疫应答等生理过程，其水平变化与神经退行性疾病、慢性疼痛综合征及精神类疾病的研究密切相关。该试剂盒通过特异性抗体对实现目标蛋白的高灵敏度捕获与检测，配套标准品可建立精确的定量曲线，支持科研人员对中枢神经系统、内分泌系统及相关病理模型的深入分析。样本兼容性涵盖体液与组织裂解液，特别适用于跨样本类型的对比研究，例如脑脊液与血清中β-EP的动态关联分析，或特定脑区组织匀浆的局部浓度检测。实验流程严格遵循标准化操作，配套试剂完整提供，确保实验数据可重复性，为探索β-EP在神经信号传导、应激适应机制及药物干预效应等方向提供可靠工具。
别名：

beta-Endorphin, beta-EP
缩写：

β-EP
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	99
	Range %	91-110
1:4	Average %	97
	Range %	92-105
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.537	2.568	2.553	2.443
2000	2.010	1.999	2.005	1.895
1000	1.600	1.532	1.566	1.456
500	1.032	1.122	1.077	0.967
250	0.567	0.600	0.584	0.474
125	0.391	0.410	0.401	0.291
62.5	0.242	0.254	0.248	0.138
0	0.109	0.111	0.110

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Measuring of serum taurine and beta endorphin in women as a pre-early marker for diagnosis of cancer breast SA Dardier,/,2023
Effects of circuit-type resistance training on industrial opiate induced neurotransmitter disruption and performance changes of previously addicted men H Arazi,Biomedical Human Kinetics,2020
In major depression, increased kappa and mu opioid receptor levels are associated with immune activation Al-Hakeim H K, et al,Acta Neuropsychiatrica,2019
In Major Depression, Increased Serum Dynorphin and Kappa Opioid Receptor Levels are Positively Associated with Mu Opioid Receptor Levels and Immune Activation and Are Attenuated by Nicotine Dependence Hussein Al-Hakeim, et al,Preprints,2019
Development of a Novel Neuro-Immune and Opioid-Associated Fingerprint with a Cross-Validated Ability to Identify and Authenticate Unknown Patients with Major Depression: Far Beyond Differentiation, Discrimination and Classification Hussein Al-Hakeim, et al,Preprints,2019
IL-10 is associated with increased mu-opioid receptor levels in major depressive disorder Al-Fadhel SZ, et al,European Psychiatry,2019
Sweating away depression? The impact of intensive exercise on depression. Balchin R.et al,J Affect Disord.,2016
Hypomagnetic field alters circadian rhythm and increases algesia in adult male mice MO Wei-Chuan. et al,Progress in Biochemistry and Biophysics,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.