Horse Beta-Endorphin,β-EP ELISA Kit

产品详情

产品描述：

马β内啡肽(β-EP)酶联免疫试剂盒（CSB-E06831Eq）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的β-EP含量。β-EP是重要靶点。它属内源性阿片肽，广泛分布于中枢和外周。其参与痛觉调节、情绪调控等生理过程。研究机制上，主要通过与阿片受体结合发挥作用，深入探索其机制有助于开发治疗疼痛、精神疾病等的药物。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域探究马科动物在应激状态、慢性疼痛模型、神经系统疾病或免疫调节研究中的β-EP动态变化，为揭示其生理功能及分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

beta-Endorphin, beta-EP
缩写：

β-EP
种属：

Equus caballus (Horse)
样本类型：

serum, plasma
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-111
1:2	Average %	105
	Range %	102-109
1:4	Average %	93
	Range %	90-97
1:8	Average %	88
	Range %	85-93

回收率：

The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-105
EDTA plasma (n=4)	91	90-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.