Bovine Beta-Endorphin,β-EP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛β内啡肽(β-EP)酶联免疫试剂盒（CSB-E13051B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的β-EP含量。β - EP即β - 内啡肽，是人体自身产生的具有强大镇痛作用的内源性活性物质。其研究机制主要围绕它与体内阿片受体结合，调节痛觉传导、情绪等生理过程，在镇痛、精神疾病等领域研究较多，对理解人体生理和开发相关疗法有重要意义。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，可为神经生物学、疼痛机制研究或应激反应动物模型提供数据支持，例如探究药物干预、环境刺激对β - EP表达水平的影响；适用于牛源样本的体外定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

beta-Endorphin, beta-EP
缩写：

β-EP
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

46.88 pg/mL-3000 pg/mL
灵敏度：

11.72 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	84
	Range %	80-88
1:4	Average %	92
	Range %	88-97
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.