Rabbit von Willebrand Factor,VWF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫法定量检测兔血清或血浆样本中的血管性血友病因子/瑞斯托霉素辅因子（VWF），该蛋白在止血过程中介导血小板与血管内皮黏附，其表达异常与血管性血友病及血栓性疾病相关，是凝血功能研究的重要指标。试剂盒灵敏度下限为0.312 ng/mL，线性范围上限达20 ng/mL，适用于低丰度样本的精准检测，实验全程可在4-6小时内完成。通过预包被的高特异性抗体实现目标蛋白捕获，配合优化的封闭体系降低非特异性结合，确保检测结果的重复性与可靠性。该产品广泛应用于心血管疾病动物模型中的凝血机制研究、抗血栓药物体外作用评估以及血小板功能相关的基础科研实验，可为血管内皮损伤、血栓形成等病理过程的分子机制探索提供数据支持，满足实验室对VWF蛋白定量分析的需求。
缩写：

VWF
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	87-106
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rabbit VWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-103
EDTA plasma (n=4)	94	86-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.