Pig von Willebrand Factor,vWF ELISA Kit

Pig von Willebrand Factor,vWF ELISA Kit

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中文名称：

猪血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒
货号：

CSB-E09395p
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO猪血管性血友病因子/瑞斯托霉素辅因子（VWF）酶联免疫检测试剂盒（货号CSB-E09395p），采用竞争法原理实现定量检测，适用于猪血清或血浆样本中VWF蛋白的科研分析。VWF是一种参与止血过程的重要糖蛋白，通过介导血小板与血管损伤部位的黏附及稳定凝血因子VIII发挥作用，其瑞斯托霉素辅因子活性是评估血小板功能的关键指标。本试剂盒检测灵敏度高，标准曲线范围覆盖0.625-40 ng/mL，实验仅需100μL样本即可完成检测，操作流程包含预包被板、生物素标记抗体及HRP-亲和素反应体系。该产品可应用于猪凝血机制研究、出血性疾病动物模型建立、兽用止血药物开发评估，或畜牧养殖中猪群凝血功能的群体健康监测等科研场景，为探究VWF在生理病理过程中的调控机制提供可靠工具。
别名：

VWF ELISA kit; F8VWFvon Willebrand factor ELISA kit; vWF ELISA kit; Fragment ELISA kit
缩写：

VWF
Uniprot No.：

Q28833
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-99
1:200	Average %	98
	Range %	92-104
1:400	Average %	89
	Range %	82-95
1:800	Average %	88
	Range %	82-94

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

0.084

0.085

0.177

0.174

0.176

0.365

0.375

0.370

0.692

0.704

0.698

2.5

1.253

1.244

1.249

1.25

1.701

1.714

1.708

0.625

2.023

2.121

2.072

2.323

2.425

2.374

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
亚细胞定位：

Secreted. Secreted, extracellular space, extracellular matrix.
组织特异性：

Plasma.
数据库链接：

STRING: 9823.ENSSSCP00000000759

UniGene: Ssc.978

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.