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Rat von Willebrand Factor,vWF ELISA Kit

  • 中文名称:
    大鼠血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒
  • 货号:
    CSB-E08438r
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO专用于定量检测大鼠血清、血浆及组织匀浆样本中血管性血友病因子/瑞斯托霉素辅因子(VWF)的酶联免疫试剂盒(货号:CSB-E08438r),采用双抗体夹心ELISA原理,通过预包被特异性抗体捕获目标蛋白,结合生物素标记的检测抗体与链霉亲和素-HRP显色系统实现精准定量分析。VWF作为重要的凝血辅助蛋白,参与血小板与血管内皮细胞的黏附及凝血因子VIII的稳定,在止血与血栓形成研究中具有关键意义。本试剂盒检测范围为0.312 ng/mL至20 ng/mL,灵敏度达0.156 ng/mL,可满足不同浓度样本的检测需求,适用于心血管疾病模型构建、血栓机制研究、药物疗效评估等科研场景。实验操作简便,兼容常规酶标仪检测,提供标准品、预包被板及相关试剂全套组分,严格验证批次间稳定性,确保实验数据的可靠性与重复性。本产品仅用于科学研究,为探索VWF在生理病理过程中的作用提供高效工具。
  • 别名:
    Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-99  
    1:200 Average % 97  
    Range % 92-105  
    1:400 Average % 85  
    Range % 81-87  
    1:800 Average % 88  
    Range % 83-95  
  • 回收率:
    The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-93  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.300 2.488 2.394 2.257  
    10 2.011 2.102 2.057 1.920  
    5 1.446 1.560 1.503 1.366  
    2.5 0.903 0.900 0.902 0.765  
    1.25 0.628 0.586 0.607 0.470  
    0.625 0.350 0.364 0.357 0.220  
    0.312 0.242 0.258 0.250 0.113  
    0 0.134 0.140 0.137    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能:
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 亚细胞定位:
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 组织特异性:
    Plasma.
  • 数据库链接: