Dog von Willebrand Factor,vWF ELISA Kit

Dog von Willebrand Factor,vWF ELISA Kit

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中文名称：

犬血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒
货号：

CSB-E12759c
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO犬血管性血友病因子/瑞斯托霉素辅因子（VWF）酶联免疫检测试剂盒（货号：CSB-E12759c），采用双抗体夹心法定量分析犬血清、血浆或组织匀浆样本中的VWF蛋白浓度。VWF是参与血小板黏附和凝血因子Ⅷ稳定的关键糖蛋白，其表达水平与犬类凝血功能异常及遗传性出血性疾病密切相关，是研究犬类凝血机制的重要分子标记物。试剂盒检测灵敏度达0.625 ng/mL，线性范围为0.625-40 ng/mL，操作流程包括标准品与样本的孵育、洗涤及显色步骤，仅需50 μL样本即可完成检测，预包被96孔板支持灵活拆分使用。该产品适用于科研场景中犬类VWF蛋白表达水平的定量分析，可用于探究凝血相关疾病的分子机制、评估实验动物模型的血栓或出血性病理状态，以及筛选影响凝血功能的药物或化合物研究，为兽医基础研究与生物医学实验提供可靠工具。
别名：

VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
缩写：

VWF
Uniprot No.：

Q28295
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.32 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	101
	Range %	95-107
1:200	Average %	94
	Range %	90-100
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

回收率：

The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.093	0.101	0.097
20	0.209	0.216	0.213
10	0.422	0.441	0.432
5	0.739	0.715	0.727
2.5	1.168	1.124	1.146
1.25	1.602	1.709	1.656
0.625	1.779	1.879	1.829
0	2.170	2.297	2.234

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
基因功能参考文献：
1. only the c.1657G variant was found in the homozygous state exclusively in Von Willebrand disease affected dogs, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer and German Shorthaired Pointer breeds PMID: 28696025
2. the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus PMID: 17593818
3. Expression of mutant VWF proteins confirmed that VWF multimerization is not necessary for Weibel-Palade body creation PMID: 15331450
相关疾病：

Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF.
亚细胞定位：

Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
组织特异性：

Plasma.
数据库链接：

KEGG: cfa:399544

STRING: 9615.ENSCAFP00000022453

UniGene: Cfa.111

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.