Rabbit von Willebrand Factor,VWF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒（CSB-E08525Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的VWF含量。VWF（血管性血友病因子）在止血和血栓形成中起关键作用。其背景上，VWF缺陷或异常会导致血管性血友病。研究机制方面，它能与血小板和内皮下胶原结合，介导血小板黏附、聚集，还参与凝血因子Ⅷ的运输和保护，维持其活性。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品广泛应用于心血管疾病动物模型中的凝血机制研究、抗血栓药物体外作用评估以及血小板功能相关的基础科研实验，可为血管内皮损伤、血栓形成等病理过程的分子机制探索提供数据支持，满足实验室对VWF蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

VWF
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	87-106
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rabbit VWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-103
EDTA plasma (n=4)	94	86-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.