Rabbit Immunoglobulin A,IgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测兔免疫球蛋白A（IgA）的竞争法ELISA试剂盒，适用于分析血清、血浆、细胞培养上清及组织匀浆等多种样本类型，最低检测限为12 ng/mL，线性检测范围为12-3000 ng/mL。兔IgA是黏膜免疫系统的重要组成部分，广泛参与呼吸道、消化道等黏膜屏障的免疫防御，其水平变化可反映机体局部免疫应答状态。该试剂盒基于抗原-抗体特异性结合原理，通过预包被靶标结合物与样本中IgA竞争结合标记抗体，结合显色强度与目标物浓度呈负相关的特性实现精确定量。其宽泛的检测范围可兼容不同浓度样本的检测需求，尤其适用于疫苗研究、肠道菌群互作实验或慢性炎症模型中黏膜免疫效能的评估，也可用于体外细胞实验探究免疫调节因子对IgA分泌的影响。试剂盒包含预包被板、标准品、检测抗体及全套实验缓冲液，操作流程标准化，兼容常规实验室设备，为科研人员提供便捷可靠的IgA定量分析工具。
缩写：

IgA
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

12 ng/mL-3000 ng/mL
灵敏度：

3 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	84-97
1:2000	Average %	91
	Range %	89-96
1:4000	Average %	93
	Range %	87-104
1:8000	Average %	92
	Range %	86-98

回收率：

The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-103
EDTA plasma (n=4)	95	85-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.