Mouse Immunoglobulin A, IgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠免疫球蛋白A（IgA）酶联免疫检测试剂盒（货号：CSB-E07986m），采用竞争法原理实现精确定量，适用于血清、血浆样本中IgA的浓度检测。IgA作为黏膜免疫系统的重要效应分子，在呼吸道、消化道等部位的免疫防御中发挥关键作用，其水平变化可反映机体免疫状态及特定病理进程。试剂盒标准曲线覆盖25-1600 ng/mL的检测范围，可灵敏检测微量样本中的IgA含量，适用于肠道菌群研究、呼吸道感染模型评估、免疫调节剂药效分析等科研场景。实验操作采用预包被板条与生物素化检测体系，通过标准品与样本中IgA竞争结合抗体的特性实现定量分析，配套提供标准品、检测抗体及显色底物等完整组分。该试剂盒特别适用于小鼠感染性疾病模型、自身免疫疾病研究或过敏反应实验中黏膜免疫水平的动态监测，为免疫机制探索及生物标志物分析提供可靠工具。
缩写：

IgA
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

25 ng/mL-1600 ng/mL
灵敏度：

25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	93
	Range %	88-95
1:10000	Average %	95
	Range %	90-99
1:20000	Average %	89
	Range %	85-94
1:40000	Average %	97
	Range %	91-102

回收率：

The recovery of mouse IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	102	98-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.