Mouse Beta-Endorphin,β-EP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠β内啡肽(β-EP)酶联免疫试剂盒（CSB-E06827m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的β-EP含量。β-EP即β-内啡肽，是内源性阿片肽家族中的重要成员，广泛分布于中枢神经系统和外周组织。其研究机制主要围绕它与阿片受体结合，在痛觉调制、神经内分泌调节、情绪调控等方面发挥关键作用，对了解机体生理和病理过程意义重大。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于基础科研领域，如探究中枢神经系统疾病模型、应激反应机制或镇痛药物研究中β-EP的动态变化，亦可用于评估动物模型中组织特异性β-EP表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

beta-Endorphin, beta-EP
缩写：

β-EP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.195 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	95
	Range %	91-98
1:4	Average %	101
	Range %	97-104
1:8	Average %	92
	Range %	89-95

回收率：

The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	94	90-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.