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中文名称:猴脂联素(ADP)酶联免疫试剂盒
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货号:CSB-EL001366Mk
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:本试剂盒(货号:CSB-EL001366Mk)采用双抗体夹心酶联免疫吸附法(ELISA)定量检测猴血清、血浆或组织匀浆样本中的脂联素(ADP)水平。脂联素是一种由脂肪细胞分泌的蛋白类激素,在调节胰岛素敏感性、糖脂代谢平衡及抗炎过程中发挥重要作用,其表达水平与肥胖、糖尿病等代谢性疾病密切相关。试剂盒检测灵敏度为0.45 ng/mL,线性范围为0.45-30 ng/mL,实验需使用标准品梯度稀释进行定量分析。操作流程中通过特异性捕获抗体和检测抗体的双重识别确保高特异性,适用于科研领域中对非人灵长类动物模型的代谢机制研究,例如评估肥胖干预措施、探究糖尿病病理进程或分析药物对脂联素通路的影响。试剂盒包含预包被96孔板、标准品、检测抗体及显色底物等核心组分,所有组分需在-20°C条件下分装冻存以保持稳定性,避免反复冻融。CUSABIO科研用户提供可靠的检测工具,支持代谢性疾病基础研究及药物开发中的生物标志物分析需求。
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缩写:ADP
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种属:Monkey
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.45 ng/mL-30 ng/mL
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灵敏度:0.11 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 30 2.481 2.407 2.444 2.358 15 1.876 1.862 1.869 1.783 7.5 1.271 1.265 1.268 1.182 3.75 0.715 0.726 0.721 0.635 1.8 0.492 0.484 0.488 0.402 0.9 0.288 0.301 0.295 0.209 0.45 0.212 0.226 0.219 0.133 0 0.083 0.089 0.086 -
数据处理:
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货期:3-5 working days
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